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So, manager Jean Tigana will select from the squad that secured a 2-2 draw in the first leg in Italy, where Hayles' two-match ban did not apply.
The game at Loftus Road has been billed as one of the most important nights in Fulham's history and a big crowd is expected, with Bologna certain to attract a sizeable following from London's Italian community.
"It promises to be a fantastic occasion. It's one that every Fulham fan should be there to share," said Fulham's club's chief executive officer Bruce Langham.
"I think that the atmosphere at Loftus Road will be fantastic. It will be full of Fulham fans making a huge amount of noise.
"The Italians certainly gave us a hot reception when we went to Bologna, so I'm hoping we repay them with interest."
Fulham (from): Van der Sar, Finnan, Melville, Brevett, Goma, Malbranque, Legwinski, Davis, Boa Morte, Marlet, Saha, Inamoto, Sava, Collins, Hayles, Taylor, Ouaddou, Knight, Leacock.